Brine Shrimp Hatching Experiment

Brine Shrimp Hatching ExperimentWherever flavour water is evaporated on a large scale, or salt lakes develop, sop runt will eventu completelyy appear. How do they line up there? Certain birds visit salt waters shorebirds such as gulls and stilts, for example. Could they transport the adult do persona prawn or eggs? Could drench shrimp eggs travel by wind?An interesting fact to remember is that although sop shrimp bugger off very well under artificial conditions, brine shrimp atomic cast 18 not found in the open ocean. This is because the brine shrimps only defense instrument against predators (fish and other(a) invertebrates) is hyper-saline bodies of water.For this reason, brine shrimp make up developed the most efficient osmo-regulatory system in the animal kingdom. Ask the pupils to provide an explanation of why brine shrimp are present only in salt ponds and soda lakes and not in the ocean. expend a glass container as a crosshatch tank for the brine shrimp, either a wide-mouth quart jar or a shallow glass pan at least two inches chummy (this will work best). Fill the container with mavin quart of salt-water outcome mix 1 to1-1/2 teaspoons of sea salt mixture or non-iodized table salt per cup of bottled water. (If you want to use tap water, let it sit for an hour so the chlorine settles. You can also use rock or fish tank salt.) The shrimp will die in salt water that is either too weak or too strong.Sprinkle to the highest degree one sixteenth of a teaspoon of brine shrimp eggs into the hit you dont need to cover much than one square inch on the surface of the water. turn over the container in a room where bright sunlight can reach it. Your brine shrimp should start hatching in just 24 hoursThe shrimp will live 1-3 days without food. If you want to keep them longer for a more than in-depth study, feed them a very tiny occur of yeast a few grains as needed. You might also need to change the water occasionally, if it gets cloudy. Clea n out unhatched eggs from the top of the container, which will allow more oxygen to get into the water.Observing Brine ShrimpYou can study your brine shrimp close up with a magnifying glass, stereo microscope, or conf utilize microscope. Use a pipet or medicine dropper to catch some of the shrimp and transfer them with sufficient water into a petri dish for well-situated observation. Look at them closely with low power (10-30x) magnification. What parts of the brine shrimp can you identify? What are their swimming habits? Eating habits? How do they use their phyllopods? How do they respond to light? If you can, compare the larval stage with the adult stage. Keep track of your observations in a notebook and include sketches of the shrimp.Learn intimately the effects of the surrounding conditions on brine shrimp To start, test the pH level in the brine shrimps tank water ideal conditions are a pH of around 8, but no lower than 5 and no higher than 10. Use pH paper for the test. To r aise the pH level in the tank, add a little bit of baking soda.Discover more with a project where you change the tank environment by adding pollutants. Transfer about an equal number of brine shrimp to several petri dishes to be your test samples. Try adding 1-3 drops of a different reply to the water in each petri dish vegetable oil, soap, vinegar, ammonia, or anything else that comes to mind. Observe the samples at low power magnification and record whats going on. How do the pollutants affect the sample? Is there a remnant visible in twenty minutes? One hour? Three? How might you counteract the pollutants?You can also try hatching several batches of shrimp at a time, using different hatchery conditions for each batch. Fill 3-4 petri dishes with different solutions you might use plain tap water, water with a low pH (acidic), and regular salt water to be the control that you can compare the effects to. Before you start, hypothesize which solution will have the best results and w hich will have the worst. Sprinkle a small amount of eggs into each dish. After 24 hours, check on the dishes again. Has anything happened? What are the results after 48 hours? 72 hours? Use a magnifying glass for your observations, and make sketches. Were you right about which solutions would work best and worst? How do you think factors such as temperature (colder or warmer) or more or less light might affect the hatching success rate of the brine shrimp?PROBLEM STATEMENTWhat is the essence number of fortunate hatching of brine shrimp?HYPOTHESISThe number of hatching is the most at the temperature of 30 C. number of hatching is the lowest at 34 C.VARIABLESManipulated temperature of incubationResponding number of eggs hatchFix concentration of salt solution, number of eggsAPPARATUS AND MATERIALSBrine shrimp cysts, 25ml salt, 100cm dechlorinated water, 40cm beaker of salt water, 100cm beakers, water baths of temperature 30 C and 34 C, stirring rod, forceps, pipette, microscope , saptula of eggsPROCEDUREPlace 25ml of sea salt into a 100cm beaker.100cm of de-chlorinated water and stir until the salt is completely dissolved.The beaker is labeled with the group name, variety and the temperature in which it will be tested. saptula of eggs is added into the beaker.Placed the beaker in incubator of temperature 30 C, 34 C and at room temperature. The cysts are left for one night.On the following day, the cysts is calculated. Stir the solution containing the cysts gently to make sure they are evenly distributed. 0.5 cm of the solution is pipetted and edit into Petri dish.Calculate the total amount of the cysts which is hatched and unhatched at all temperature under light microscope. The experiment is repeated three times to get the average value of the amount calculated.all the values calculated is multiplied by 50 to get the total amount of brine shrimp in 25ml of solution.DISCUSSIONAccording to the tabulated data above, total cysts hatched is the highest at temperature of 30 C because higher temperature is needed to make the surrounding warmer and suitable for hatching. The lowest eggs hatch being preserve is at temperature of 34 C because the temperature is too high. The eggs, being the enzyme might be denatured at this point and most eggs do not hatch. at that place are more eggs that has not been hatch compared to those which has hatch. This might be due to the short term experiment. The eggs are allowed to soak in the solution for only a day and most of them have not hatch yet.Conducting this experiment has risen up a few conflict and ethical issue. The cysts which has and has not hatched will be thrown after the experiment end. For public, it is not ethical to kill animal which is still alive and used as a study purpose. Although they are tiny but they do play their part in food chain. They dont have right to live freely as other organism do.But, for scientists, conducting an experiment on them may bring good advantages to hum an. Human will get the beneficiary as new medicine and discovery is notice without involving any human life in the research. People doesnt put too much attention when small animal like bribe shrimp is used in the experiment.LIMITATIONThe size of brine shrimp eggs are too small and almost impossible to be counted manually. Hence, only spatula of the eggs are used in approximation. But there is limitation in using approximation. The number of cysts used is not the same in each test tubes makes the result. This makes the result less reliable. A larger number of cysts is needed because the results of experiment may vary and by using big group of sample, the result may be more reliable.There might be some mistakes while calculating the number of cysts that has hatched or not because the number of eggs per o.5ml is a dowery and to calculate them under light microscope is almost impossible. Some students taking the number of which can be seen under lense, some takes average. The likelih ood to get the real number is low.In counting the number of the eggs, the average is taken. Only 0.5ml out of 25ml is being used to be observed under the microscope. The distribution of the eggs in the solution might not be the same even after it has been stirred using glass rod.SOURCE OF ERRORThe test tube which should be put under room temperature is being put in the laboratory which has air conditioner. This makes the temperature of surrounding lower than the room temperature and affect the result of experiment in make a conclusion that the earth is facing global warming. Hence, we are not sure whether or not, the room temperature has risen.Since they are too small, somehow, their hatched eggs are counted as unhatched eggs. This happens as there are no big difference in structure of the hatched and unhatched eggs. This may lead to victimize counting of the result and will affect the experiment.PRECAUTION STEPBe careful when pipetting the cysts because they are so small and migh t be easily get hurt. They need to be handled with care and gently.use a low light power while using microcopeas higher temperature might gives effect on the brine shrimp.CONCLUSIONThe hatching success of the brine shrimp is the highest at 34 C